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Things you can try:

• Ensure that the NTFS permissions for the web.config file are correct and allow access to the Web servers machine account.
• Check the event logs to see if any additional information was logged.
• Verify the permissions for the DLL.
• Install the .NET Extensibility feature if the request is mapped to a managed handler.
• Create a tracing rule to track failed requests for this HTTP status code. For more information about creating a tracing rule for failed requests, click

  Detailed Error Information:

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